Nuclear residence half-lives of in vitro-transcribed RNA microinjected into oocyte

Range ~1 hour
Organism African clawed frog Xenopus laevis
Reference Audibert A, Weil D, Dautry F. In vivo kinetics of mRNA splicing and transport in mammalian cells. Mol Cell Biol. 2002 Oct22(19):6706-18. doi: 10.1128/MCB.22.19.6706-6718.2002 p.6706 right column bottom paragraphPubMed ID12215528
Primary Source Jarmolowski, A., W. C. Boelens, E. Izaurralde, and I. W. Mattaj. 1994. Nuclear export of different classes of RNA is mediated by specific factors. J. Cell Biol. 124: 627-635.PubMed ID7509815
Comments P.6706 right column bottom paragraph:"Transport has been mostly studied by microinjection of in vitro-transcribed RNA into Xenopus laevis oocytes. While this approach has been useful for a biochemical characterization of the transport process, nuclear residence half-lives of the order of 1 h have been reported in most studies (primary source). Again, such a slow kinetics of export cannot represent the in vivo situation."
Entered by Uri M
ID 112186