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1 read per kilobase per million [rpkm] in helper T [Th2] cell corresponds to

Range	~1 mRNA/cell
Organism	Mouse Mus musculus
Reference	Hebenstreit D, Fang M, Gu M, Charoensawan V, van Oudenaarden A, Teichmann SA. RNA sequencing reveals two major classes of gene expression levels in metazoan cells. Mol Syst Biol. 2011 Jun 7 7: 497. doi: 10.1038/msb.2011.28. p.4 right column 3rd paragraphPubMed ID21654674
Method	RNA sequencing (RNA-seq) & RNA-FISH [Fluorescence in situ hybridization]
Comments	"As the RPKM [Read Per Kilobase per Million] as measured by RNA-seq should be proportional to the mean mRNA numbers per cell, [researchers] can use the RNA-FISH results to estimate how our RPKM values translate into mRNA numbers. [They] find that one RPKM corresponds to an average of roughly one transcript per cell in [their] Th2 data set (Figure 3C). Please note that the value of one RPKM/one transcript on average per cell serves as an estimate only as it is based on a limited number of data points. See Supplementary Figure S14 for log-transformed versions of Figure 3A–C."
Entered by	Uri M
ID	111141