Total number of mRNAs per cell

Range ~41,000 mRNAs/cell
Organism Fission yeast Schizosaccharomyces pombe
Reference Marguerat S et al., Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells. Cell. 2012 Oct 26 151(3):671-83. doi: 10.1016/j.cell.2012.09.019. p.672 right column 2nd paragraphPubMed ID23101633
Method p.672 left column 2nd paragraph:"[Investigators] acquired quantitative expression data relative to absolutely calibrated standards for transcriptomes and proteomes of haploid fission yeast cells. For transcripts, genome-wide measurements were obtained by calibrating RNA-seq data from total RNA preparations with data on absolute cellular concentrations for 49 mRNAs, covering the dynamic expression range."
Comments p.672 right column 2nd paragraph:"In proliferating cells, [investigators] measured a total of ~41,000 mRNA molecules/cell on average, representing ~5% of the overall ~802,000 rRNAs/cell in [their] samples. Protein-coding genes produced a median of 2.4 mRNA copies/cell, ranging from ~0.01 to >810 copies (Figure 1A)."
Entered by Uri M
ID 111633