| Comments |
"A major leap forward in the analysis of nucleoporins and
NPC structure was the result of a convergence of two lines of
research. First, completion of the yeast genome made it possible
to identify likely NPC proteins by sequence similarity to
known nucleoporins. Second, purification of the yNPC [yeast NPC] by
Rout and colleagues made possible the potential identification
of all pore-complex proteins by a proteomics analysis
[2,27]. Surprisingly, they found that the yNPC is composed
of only about 30 proteins, far fewer than the original estimates,
with each protein present in 8 to 56 copies per pore." |