| Comments |
"Although the above experiments clearly demonstrate that the
firing state of neurons has a large influence on ATP consumption,
the simple absence or presence of TTX [Tetrodotoxin] is a relatively blunt tool
for examining the impact of activity. [Researchers] therefore examined
ATP levels in synapses during defined bursts of electrical activity. [They] monitored Syn-ATP [a quantitative genetically encoded
optical reporter of presynaptic ATP] over a 10 min period during which
neurons were stimulated with a 60 s burst of 10 Hz firing (in the
absence of TTX)." |