| Range |
Table - link
|
| Organism |
Bacteria Bacillus subtilis |
| Reference |
Sharpe ME, Hauser PM, Sharpe RG, Errington J. Bacillus subtilis cell cycle as studied by fluorescence microscopy: constancy of cell length at initiation of DNA replication and evidence for active nucleoid partitioning. J Bacteriol. 1998 Feb180(3):547-55. p.551 table 2PubMed ID9457856
|
| Primary Source |
See (ref 15) beneath table |
| Method |
Light microscopic methods. "Media and growth conditions: S medium contained (NH4)2SO4 (0.2% w/v),
K2HPO4 (1.4%), KH2PO4 (0.6%), sodium citrate (0.1%), Mg2SO4 (0.02%),
MnSO4 (0.056%), and glucose (0.5%). TS medium was S medium supplemented
with L-glutamate (0.5%) and Difco yeast extract (0.001%). CH medium (which
contains 10% casein hydrolysate) was as specified in detail previously (32a
modified as described in reference 35). CHG medium was CH medium containing
glucose (0.5%). All media were supplemented with tryptophan (20 mg/ml)." |
| Comments |
"To determine the average cell cycle for B. subtilis cells
growing in various media, affording generation times between
30 and 73 min, [researchers] used the light microscopic methods of
Hauser and Errington (15). Some of the data for one medium,
CH, were described previously (15). In Fig. 1 and 2, data from
200 or more individual cells growing in each of the four different
media are summarized. For each cell the state of the
nucleoids (number and conformation) were compared with
cell length and DNA content per cell (see Materials and Methods).
To check the accuracy of the fluorimetric DNA estimations,
[researchers] measured the DNA concentration of each culture by
a direct chemical method and, having determined the cell
number by direct microscopic counting, calculated the average
DNA content per cell. As shown in Table 2, the values obtained
differed only by about 10% from the average DNA
content per cell obtained by fluorescence microscopy. An error
of this magnitude would have little effect on the cell cycle
modelling described below." |
| Entered by |
Uri M |
| ID |
111070 |