| Range |
~100 min^-1
|
| Organism |
Bacteria Bacillus subtilis |
| Reference |
Breaker RR. Riboswitches and the RNA world. Cold Spring Harb Perspect Biol. 2012 Feb 1 4(2). pii: a003566. doi: 10.1101/cshperspect.a003566. p.4 right column bottom paragraphPubMed ID21106649
|
| Primary Source |
Brooks KM, Hampel KJ. 2009. A rate-limiting conformational step in the catalytic pathway of the glmS ribozyme. Biochemistry 48: 5669–5678. doi: 10.1021/bi900183r.PubMed ID19449899
|
| Comments |
"One of the most interesting mechanisms for riboswitch mediated
gene control integrates ligand binding and ribozyme
activities (Fig. 2F). Representatives of the glmS riboswitch
class function as metabolite-responsive self-cleaving
ribozymes (Winkler et al. 2004). The ligand for this ribozyme-riboswitch is glucosamine-6-phosphate (GlcN6P),
which is the metabolic product of the protein whose expression
is controlled by the RNA. A glmS ribozyme construct
from Bacillus subtilis cleaves with an GlcN6P-induced rate
constant of nearly 100/min (primary source),
which is an enhancement of more than 1 billion-fold above
the rate constant for spontaneous RNA cleavage by phosphoester
transfer. Ribozyme self-cleavage initiates rapid destruction
of its associated mRNA coding region via selective
nuclease degradation (Collins et al. 2007)." |
| Entered by |
Uri M |
| ID |
110860 |