Binding constant of riboswitch to flavin mononucleotide (FMN)

Range ~5 nM
Organism Bacteria Bacillus subtilis
Reference Lee ER, Blount KF, Breaker RR. 2009. Roseoflavin is a natural antibacterial compound that binds to FMN riboswitches and regulates gene expression. RNA Biol 6: 187–194. p.2 right column top paragraphPubMed ID19246992
Comments "An apparent dissociation constant (KD) of ~100 nM was estimated from the in-line probing data generated in the presence of roseoflavin (Fig. 1C and D). Compared to the KD values previously established for the 165 ribD RNA (refs 5,10), the aptamer has a stronger affinity for FMN (KD ~5 nM) than roseoflavin, whereas riboflavin (KD ~3 µM) binds less tightly than the antimetabolite (Fig. 1D). These findings suggest that the dimethylamino group on the flavin ring of roseoflavin (Fig. 1B) permits more favorable binding to the aptamer than the methyl group it replaces."
Entered by Uri M
ID 110864