| Range |
~1 µM
|
| Organism |
Bacteria Bacillus subtilis |
| Reference |
Sudarsan N, Wickiser JK, Nakamura S, Ebert MS, Breaker RR. An mRNA structure in bacteria that controls gene expression by binding lysine. Genes Dev. 2003 Nov 1 17(21):2688-97. Abstract & p.2692 right column top paragraphPubMed ID14597663
|
| Method |
"A Scatchard plot also was created by using data from a series of equilibrium dialysis experiments conducted under various concentrations of tritiated L-lysine (Fig. 4D).
The slope of the resulting line indicates that the 179 lysC RNA binds to L-lysine with an apparent KD of
~1µM, which is consistent with that observed by using
in-line probing. Furthermore, the x-intercept of the line
occurs near an r value of one, which demonstrates that
the RNA forms a 1:1 complex with its ligand." |
| Entered by |
Uri M |
| ID |
110869 |