Binding constant of riboswitch to lysine

Range ~1 µM
Organism Bacteria Bacillus subtilis
Reference Sudarsan N, Wickiser JK, Nakamura S, Ebert MS, Breaker RR. An mRNA structure in bacteria that controls gene expression by binding lysine. Genes Dev. 2003 Nov 1 17(21):2688-97. Abstract & p.2692 right column top paragraphPubMed ID14597663
Method "A Scatchard plot also was created by using data from a series of equilibrium dialysis experiments conducted under various concentrations of tritiated L-lysine (Fig. 4D). The slope of the resulting line indicates that the 179 lysC RNA binds to L-lysine with an apparent KD of ~1µM, which is consistent with that observed by using in-line probing. Furthermore, the x-intercept of the line occurs near an r value of one, which demonstrates that the RNA forms a 1:1 complex with its ligand."
Entered by Uri M
ID 110869