| Range |
≈0.95 unitless
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Famili I, Forster J, Nielsen J, Palsson BO. Saccharomyces cerevisiae phenotypes can be predicted by using constraint-based analysis of a genome-scale reconstructed metabolic network. Proc Natl Acad Sci U S A. 2003 Nov 11 100(23):13134-9. p.13135 right column 2nd paragraphPubMed ID14578455
|
| Primary Source |
[28] Verduyn, C., Stouthamer, A. H., Scheffers, W. A. & van Dijken, J. P. A theoretical evaluation of growth yields of yeasts. (1991) Antonie Leeuwenhoek 59, 49–63.PubMed ID2059011
|
| Method |
Primary source abstract: "Growth yields of Saccharomyces cerevisiae and Candida utilis in carbon-limited chemostat cultures were evaluated... In vivo P/O-ratios can be calculated for aerobic growth on ethanol and acetate, provided that the gap between the theoretical and experimental ATP requirements as observed for growth on glucose is taken into account. This was done in two ways: via the assumption that the gap is independent of the growth substrate (i.e. a fixed amount of ATP bridges the difference between the theoretical and experimental values). alternatively, on the assumption that the difference is a fraction of the total ATP expenditure, that is dependent on the substrate." |
| Comments |
P.13135 right column 2nd paragraph: "The in silico model can be used to assess network properties such as the P/O ratio and energy maintenance costs and to compute whole-cell functions. The efficiency of aerobic respiration is measured by the P/O ratio. Experimental studies of isolated
mitochondria have shown that S. cerevisiae lacks site I proton
translocation (primary source). Consequently, estimation of the maximum
theoretical or ‘‘mechanistic’’ yield of the ETS [Electron Transport System] alone gives a P/O ratio of 1.5 for oxidation of NADH in S. cerevisiae grown on glucose (primary source). However, based on experimental measurements, it has been determined that the net in vivo P/O ratio is ≈0.95 (primary source)." |
| Entered by |
Uri M |
| ID |
110797 |