Maximal P/O ratio (ATP made per O atom reduced) assuming that 3H+ are required by ATP synthase to drive phosphorylation of 1 ATP

Value 2.333 unitless
Organism Bacteria Escherichia coli
Reference Sauer U, Bailey JE. Estimation of P-to-O ratio in Bacillus subtilis and its influence on maximum riboflavin yield. Biotechnol Bioeng. 1999 Sep 20 64(6):750-4. DOI: 10.1002/(SICI)1097-0290(19990920)64:6<750::AID-BIT15>3.0.CO2-S link p.752 right column top paragraph PubMed ID10417225
Primary Source Gottschalk G. 1986. Bacterial metabolism, 2nd edition. New York: Springer-Verlag.
Method Abstract: "Simultaneous growth and riboflavin overproduction were investigated using a previously developed stoichiometric model of Bacillus subtilis metabolism. A fit of model predictions to experimental data was used to obtain estimates of fundamental energetic parameters of B. subtilis." P.750 right column bottom paragraph: "A previously constructed stoichiometric model of B. subtilis metabolism was used as published (Sauer et al., 1998). Briefly, mass balances on cellular metabolites provide a set of linear equations relating intracellular and external fluxes."
Comments P.752 left column bottom paragraph: "If [investigators] assume that three protons are required by ATP synthase to drive phosphorylation of one ATP (primary source), the maximum P/O in E. coli would be 7⁄3, with NDH-I contributing one P/O unit. B. subtilis is known to contain three terminal oxidases, two of which accept electrons from menaquinone and one from cytochrome c (Fig.3)."
Entered by Uri M
ID 113248