| Range |
~1.333 (correlates to a Y[max]ATP of ~9.5 g/mol) unitless
|
| Organism |
Bacteria Bacillus subtilis |
| Reference |
Sauer U, Bailey JE. Estimation of P-to-O ratio in Bacillus subtilis and its influence on maximum riboflavin yield. Biotechnol Bioeng. 1999 Sep 20 64(6):750-4. DOI: 10.1002/(SICI)1097-0290(19990920)64:6<750::AID-BIT15>3.0.CO2-S link abstract, p. 752 right column top paragraph & p.753 right column bottom paragraph PubMed ID10417225
|
| Method |
Abstract: "Simultaneous growth and riboflavin overproduction were investigated using a previously developed stoichiometric model of Bacillus subtilis metabolism. A fit of model predictions to experimental data was used to obtain estimates of fundamental energetic parameters of B. subtilis." P.750 right column bottom paragraph: "A previously constructed stoichiometric model of B. subtilis
metabolism was used as published (Sauer et al., 1998). Briefly, mass balances on cellular metabolites provide a set of linear equations relating intracellular and external fluxes." |
| Comments |
Abstract: "Although multiple solutions describe the experimental data, evidence for a P-to-O ratio of about 1(1/3) mole of ATP produced per atom of oxygen consumed in oxidative phosphorylation was provided by genomic analysis of electron transport components, because no homologue of the proton-translocating NADH dehydrogenase I was found in the B. subtilis genome database." P.752 right column top paragraph: "Thus, in glucose-grown B. subtilis, the maximum P/O appears to be 4⁄3, a value that compares very well with the overall respiratory H+/e− stoichiometry of 1.9 determined from oxygen pulse experiments with exponentially growing B. subtilis (Lauraeus and Wikstro¨m, 1993). According to Fig.2, this P/O correlates to a Y[max]ATP of about 9.5 g/mol." P.753 right column bottom paragraph: "Conclusions: The results indicate that glucose-limited wild-type B. subtilis
exhibits a P/O of about 4⁄3, a mATP of about 9 mmol ATP/g/h, and a Y[max]ATP around 9.5 g/mol (equivalent to an ATP demand of 105 mmol per gram of newly formed biomass)." |
| Entered by |
Uri M |
| ID |
113245 |