in silico P/O ratio for oxidation of NADH and FADH2 during growth on glucose

Value 1.04 unitless
Organism Budding yeast Saccharomyces cerevisiae
Reference Famili I, Forster J, Nielsen J, Palsson BO. Saccharomyces cerevisiae phenotypes can be predicted by using constraint-based analysis of a genome-scale reconstructed metabolic network. Proc Natl Acad Sci U S A. 2003 Nov 11 100(23):13134-9. p.13135 right column 2nd paragraphPubMed ID14578455
Method "The metabolic capabilities of the S. cerevisiae network were calculated by using flux balance analysis and linear optimization (14, 15, 27). For growth simulations, biomass synthesis (i.e., production of biosynthetic components at the physiological level) was selected as the objective function to be maximized, and optimization was done subject to stoichiometric, limited thermodynamics, and reaction capacity constraints by using established procedures (13–15). Optimization problems were solved by using the commercially available package LINDO (Lindo Systems, Chicago)."
Comments "In the reconstructed network, which contains no proton leakage, 12.5 molecules of ATP are generated via the ETS [Electron Transport System]. As complete oxidation of glucose leads to donation of 12 electron pairs (10 NADH and 2 FADH2) to the electron transport chain, the in silico P/O ratio is 1.04 for oxidation of NADH and FADH2 during growth on glucose, i.e., 12.5/12=1.04, agreeing well with the measured value without including any proton leakage."
Entered by Uri M
ID 110796