Value |
1.04
unitless
|
Organism |
Budding yeast Saccharomyces cerevisiae |
Reference |
Famili I, Forster J, Nielsen J, Palsson BO. Saccharomyces cerevisiae phenotypes can be predicted by using constraint-based analysis of a genome-scale reconstructed metabolic network. Proc Natl Acad Sci U S A. 2003 Nov 11 100(23):13134-9. p.13135 right column 2nd paragraphPubMed ID14578455
|
Method |
"The metabolic capabilities of the S. cerevisiae
network were calculated by using flux balance analysis
and linear optimization (14, 15, 27). For growth simulations,
biomass synthesis (i.e., production of biosynthetic components at
the physiological level) was selected as the objective function to
be maximized, and optimization was done subject to stoichiometric,
limited thermodynamics, and reaction capacity constraints
by using established procedures (13–15). Optimization
problems were solved by using the commercially available package
LINDO (Lindo Systems, Chicago)." |
Comments |
"In the reconstructed network, which contains no proton leakage, 12.5
molecules of ATP are generated via the ETS [Electron Transport System]. As complete oxidation of glucose leads to donation of 12 electron pairs (10
NADH and 2 FADH2) to the electron transport chain, the in
silico P/O ratio is 1.04 for oxidation of NADH and FADH2
during growth on glucose, i.e., 12.5/12=1.04, agreeing well
with the measured value without including any proton leakage." |
Entered by |
Uri M |
ID |
110796 |