| Reference |
Forster RE, Gros G, Lin L, Ono Y, Wunder M. The effect of 4,4'-diisothiocyanato-stilbene-2,2'-disulfonate on CO2 permeability of the red blood cell membrane. Proc Natl Acad Sci U S A. 1998 Dec 22 95(26):15815-20. p.15819 left column 2nd paragraphPubMed ID9861053
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| Comments |
"In the present case of
C18O16 exchange, the thickness assumed for the cell is the
same, D for CO2 is taken as 6.5X10^-6cm^2/sec, and
the exchange velocity constant as 44.8sec^-1. This value is
calculated from the Mills and Urey relationship (Eq. 1), a CA [carbonic anhydrase]
acceleration of 14,000, an uncatalyzed hydration velocity constant
of 0.18sec^-1, and a pH of 7.4. The average (intracellular
[CO2])/(extracellular [CO2]) is 1, so that the rate of 18O exchange
should be the same in cells and in lysate, as Barinov et al. found
(2)." |