Diffusion coefficient of sNRPs

Range 0.2-0.8 µm^2/sec
Organism Human Homo sapiens
Reference Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, Shav-Tal Y, Neugebauer KM, Stanek D. The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells. J Cell Biol. 2010 Oct 4 191(1):75-86. p.82 right column top paragraphPubMed ID20921136
Method FRAP and FCS (fluorescence correlation spectroscopy) - a technique that focuses a laser beam on a spot of interest in the nucleoplasm and monitors fluctuations in fluorescence intensity over time. The intensity record is transformed into an autocorrelation function that assesses the diffusion correlation time of the detected molecules.
Comments See BNID 105293
Entered by Uri M
ID 105697