| Range |
Table - link
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Maeder CI, Hink MA, Kinkhabwala A, Mayr R, Bastiaens PI, Knop M. Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat Cell Biol. 2007 Nov9(11):1319-26. p.1322 figure 3a & 3bPubMed ID17952059
|
| Method |
Fluorescence cross-correlation spectroscopy (FCCS) & Confocal photon-counting (APD, avalanche photodiode
detectors) imaging |
| Comments |
"Fig.3 (a) FCCS-measured fraction of Ste5 in complex with the different MAPKs. (b) Quantification of relative MAPK abundances at different sites in yeast cells using APD imaging and image analysis (see Methods). The relative abundance of the three kinases (Ste11, Ste7 and Fus3) versus the scaffold Ste5 in vegetative or pheromone stimulated cells is given at the cellular locations indicated in the schematic representations. Cells were stimulated with a-factor for 2.5–3 h. Error values indicate the s.e.m. Abundances at the shmoo tip were quantified in 20–60 cells." "Results for the MAPKs are shown as the relative molar abundances of each component versus Ste5 (Fig. 3a, b). The molar ratio of Ste11 to Ste5 was 0.2 at the shmoo tip and was, therefore, similar to that detected in the cytoplasm by FCCS (Fig. 3a, b)." |
| Entered by |
Uri M |
| ID |
110548 |