| Range |
phosphoglycerate kinase 0.056 enolase 0.049 1/hour
|
| Organism |
Bacteria Streptomyces coelicolor |
| Reference |
Trötschel C, Albaum SP, Poetsch A. Proteome turnover in bacteria: current status for Corynebacterium glutamicum and related bacteria. Microb Biotechnol. 2013 Nov6(6):708-19. doi: 10.1111/1751-7915.12035 p.712 right column top paragraphPubMed ID23425033
|
| Primary Source |
Jayapal, K.P., Sui, S., Philp, R.J., Kok, Y.J., Yap, M.G., Griffin, T.J., and Hu, W.S. (2010) Multitagging proteomic strategy to estimate protein turnover rates in dynamic systems. J Proteome Res 9: 2087–2097.PubMed ID20184388
|
| Method |
"In an effort to segregate these two underlying
mechanisms [protein synthesis and breakdown], a novel multitagging approach was
developed based on stable isotope tagging (SILAC) and
isobaric tag for relative and absolute quantification
(iTRAQ) labelling (primary source)." |
| Comments |
"Using this approach, protein degradation rates for a total
of 115 proteins could be estimated. Observed rates
were, for example, at 0.056 and 0.049 h^-1 for phosphoglycerate
kinase and enolase giving rise to the most
stable signals." |
| Entered by |
Uri M |
| ID |
110438 |