Protein degradation rate in human A549 adenocarcinoma cells

Range 2×10^-5±9×10^-7h^-1 to 5.4±0.4h^-1 (relating to half-lives of many tens of hours to just 6 min) hours^-1
Organism Human Homo sapiens
Reference Doherty MK, Hammond DE, Clague MJ, Gaskell SJ, Beynon RJ. Turnover of the human proteome: determination of protein intracellular stability by dynamic SILAC. J Proteome Res. 2009 Jan8(1):104-12. doi: 10.1021/pr800641v. p.106 left column bottom of paragraphPubMed ID18954100
Method Abstract:"In this work, [investigators] have analyzed time-dependent changes in the incorporation of a stable amino acid resolved precursor, a protocol [they] refer to as "dynamic SILAC", using 1-D gel separation followed by in-gel digestion and LC-MS/MS [Liquid chromatography–mass spectrometry] analyses to profile the intracellular stability of almost 600 proteins from human A549 adenocarcinoma cells, requiring multiple measures of the extent of labeling with stable isotope labeled amino acids in a classic label-chase experiment."
Comments P.106 left column bottom of column:"The calculated degradation rates ranged from 2×10^−5±9×10^−07h^−1 to 5.4±0.4h^−1, relating to half-lives of many tens of hours to just 6 min."
Entered by Uri M
ID 112252