Coefficient of variation of size (length) at initiation of DNA replication

Range ~20 %
Organism Bacteria Escherichia coli
Reference Nanninga, N., and Woldringh, C.L. (1985). Cell growth, genome duplication, and cell division. In Molecular Cytology of Escherichia coli, N. Nanninga, ed. (London: Academic Press), pp. 259–318. p.308 5th paragraph
Primary Source Koppes LJ, Overbeeke N, Nanninga N. DNA replication pattern and cell wall growth in Escherichia coli PAT 84. J Bacteriol. 1978 Mar133(3):1053-61 & Koppes LH, Woldringh CL, Nanninga N. Size variations and correlation of different cell cycle events in slow-growing Escherichia coli. J Bacteriol. 1978 May134(2):423-33. & Koppes LJ, Meyer M, Oonk HB, de Jong MA, Nanninga N. Correlation between size and age at different events in the cell division cycle of Escherichia coli. J Bacteriol. 1980 Sep143(3):1241-52. & Koppes LJ, Nanninga N. Positive correlation between size at initiation of chromosome replication in Escherichia coli and size at initiation of cell constriction. J Bacteriol. 1980 Jul143(1):89-99.PubMed ID346554, 350822, 6997267, 6995452
Method "This was found after autoradiography of [3H] pulse-labelled cells, which were classified according to length and in which the fraction of radioactive cells was scored for each length class."
Comments "Values for the coefficient of variation(CV) of the size (length) at initiation were on the order of 20% (Fig.31)(for details, consult primary sources)...Thus, if cell division is taken as a starting point, size at initiation of DNA replication is more accurately determined than age. (CV˜60%, BNID 110092)"
Entered by Uri M
ID 110091