Mean size of peptides generated by the proteasome measured by the acid hydrolysis method

Value 8.2 residues Range: ±1.0 Table - link residues
Organism Archaea Thermoplasma acidophilum
Reference Kisselev AF, Akopian TN, Goldberg AL. Range of sizes of peptide products generated during degradation of different proteins by archaeal proteasomes. J Biol Chem. 1998 Jan 23 273(4):1982-9. abstract and p.1985 table 2PubMed ID9442034
Method "The values in the table are means ±S.E. of at least three experiments (for the acid hydrolysis method) or two experiments (for the two rates method). In the two rates method, the mean size was determined by dividing the number of amino acids in a protein by the number of cuts plus one (see Table 1 [BNID 109949] and Fig. 1). In the acid hydrolysis method, the pooled products were hydrolyzed to amino acids, and mean peptide size was obtained by dividing the amount of amino acids after acid hydrolysis by the amount of peptides before it (Fig. 1)."
Comments "The average size of peptides generated from these four polypeptides [see table] was 8±1 residues, but ranged from 6 to 10 residues, depending on the protein, as determined by two new independent methods."
Entered by Uri M
ID 109951