Range |
~55 to 65 minutes
|
Organism |
Bacteria Escherichia coli |
Reference |
Reyes-Lamothe R, Possoz C, Danilova O, Sherratt DJ. Independent positioning and action of Escherichia coli replisomes in live cells. Cell. 2008 Apr 4 133(1):90-102. doi: 10.1016/j.cell.2008.01.044. p.93 right column top paragraphPubMed ID18394992
|
Method |
"Fluorescent C-terminal fusion-protein derivatives of E. coli
replisome components, expressed from their endogenous chromosomal promoters, were constructed and their phenotypes
assessed by growth, flow cytometry, and microscopy." |
Comments |
"Foci appear either shortly after birth or shortly before birth in
a mother cell and are present for 65 min on average. Assuming
the times of appearance and disappearance of foci mark replication
initiation and termination, respectively, then this value
for S phase, determined for timelapse cells growing on slides,
is slightly longer than [researchers'] previous estimate of 55 min for cells
growing in the same liquid medium (Wang et al., 2005 BNID 109249)." |
Entered by |
Uri M |
ID |
109252 |