Value |
0.79
nm
|
Organism |
Bacteria Escherichia coli |
Reference |
Brandt F, Etchells SA, Ortiz JO, Elcock AH, Hartl FU, Baumeister W. The native 3D organization of bacterial polysomes. Cell. 2009 Jan 23 136(2):261-71. supplementary online material p.6 top paragraphPubMed ID19167328
|
Primary Source |
Vanzi F, Takagi Y, Shuman H, Cooperman BS, Goldman YE. Mechanical studies of single ribosome/mRNA complexes. Biophys J. 2005 Sep89(3):1909-19.PubMed ID15951374
|
Method |
Primary source abstract: "A novel optical method was built to control the position of the laser trap along the microscope optical axis at 2 nm resolution, facilitating measurement of the force-extension relationship for poly(U)." |
Comments |
Supplementary online material p.6 top paragraph: "If [investigators] assume that the mRNA in such conditions (of zero external applied force) will behave as a Worm Like Chain (Kratky and Porod, 1949) with a persistence length of 0.79 nm measured experimentally for mRNA-ribosome complexes (primary source), [they] can find the number nucleotides that gives a root mean squared value of the end-to-end distance equal to 7.35 nm." |
Entered by |
Uri M |
ID |
109074 |