Backbone elasticity of mRNA-ribosome complexes

Value 900 pN Range: ±140 pN
Organism Bacteria Escherichia coli
Reference Vanzi F, Takagi Y, Shuman H, Cooperman BS, Goldman YE. Mechanical studies of single ribosome/mRNA complexes. Biophys J. 2005 Sep89(3):1909-19. p.1914 right column 2nd paragraphPubMed ID15951374
Method "A novel optical method was built to control the position of the laser trap along the microscope optical axis at 2 nm resolution, facilitating measurement of the force-extension relationship for poly(U)."
Comments "The estimated stretch modulus (K) was 870±140 pN (mean±SE,n=24), in good agreement with the values of 800–1000 pN reported for ssDNA (18)". Note-in abstract value of 900pN is given, in body of article 870pN.
Entered by Uri M
ID 109075