| Value |
30
nts/sec
Range: Table - link nts/sec
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Proshkin S, Rahmouni AR, Mironov A, Nudler E. Cooperation between translating ribosomes and RNA polymerase in transcription elongation. Science. 2010 Apr 23 328(5977):504-8. p.505 table 1 and middle column top paragraphPubMed ID20413502
|
| Method |
"To calculate the elongation rate, the
Escherichia coli culture was induced with IPTG,
and the time that elapsed between the appearance
of a specific hybridization signal from
probes complementary to the 5' and 3' segments
of the lacZ transcript was determined by dotblot
hybridization (Fig. 1 and fig. S1) (5, 6)." |
| Comments |
"The speed of transcription elongation in CH184 was remarkably
slow (only 19 nt/s), but accelerated to 30 nt/s
when Sm was added (Fig. 1C), which perfectly
matched the translation elongation rates with and
without Sm (Table 1)." |
| Entered by |
Uri M |
| ID |
108492 |