| Comments |
P.7853 right column 3rd paragraph: "If osmium tetroxide was omitted and cells were fixed in glutaraldehyde alone, it could be shown that their compartmental structure was not destroyed by fixation. That is, inulin, sucrose, and water distributions were not altered by the glutaraldehyde treatment. Furthermore, periplasmic volumes estimated from the structures depicted in electron micrographs of glutaraldehyde-fixed cells agreed with those derived from the distributions of solutes in suspensions of the living organisms. This was true for both normal and plasmolyzed cells (see Fig. 5). Such results provided the link between morphology and function which allowed the conclusion that S. typhimurium LT2 cells are composed of two compartments, cytoplasm and periplasm, each representing a substantial fraction of the bacterial cell volume." See note beneath table |