| Value |
700
molecules per cell
|
| Organism |
Bacteria Bacillus subtilis |
| Reference |
Maass S et al., Efficient, global-scale quantification of absolute protein amounts by integration of targeted mass spectrometry and two-dimensional gel-based proteomics. Anal Chem. 2011 Apr 1 83(7):2677-84. p.2681 left column 2nd paragraphPubMed ID21395229
|
| Method |
"[Researchers] combined targeted mass
spectrometry for the accurate determination of absolute protein
abundance of specific proteins with the resolving power of 2-D
PAGE to obtain a global view of a large number of protein
concentrations." |
| Comments |
"The
number of ClpC molecules in growing cells was previously
determined for B. subtilis with 1500 molecules per cell (Gerth et al., Biotechnol. 2004, 186, 179–191.) and
the study presented here yielded 700 molecules per cell, which is
well within the error tolerance of this method." |
| Entered by |
Uri M |
| ID |
108413 |