| Value |
6.17
unitless
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Preston RA, Murphy RF, Jones EW. Assay of vacuolar pH in yeast and identification of acidification-defective mutants. Proc Natl Acad Sci U S A. 1989 Sep86(18):7027-31. p.7029 right column 2nd paragraphPubMed ID2674942
|
| Method |
"...[Researchers] developed assays of vacuolar
pH using flow cytometry and fluorescence microscopy,
based on the pH-dependent fluorescence of 6-carboxyfluorescein
(6-CF)." |
| Comments |
"Cytometric analysis
of wild-type cells labeled with 6-CF showed that vacuolar pH
was maintained at 6.17 (SD = 0.07, n = 16) under several
different conditions tested." pH of vacuole varies from <5 to 6.5 according to Li et al., 2009 PMID 18786576 p.654 left column bottom paragraph |
| Entered by |
Uri M |
| ID |
108318 |