| Range |
0.72 (quenching liquid H) to 0.59 (quenching liquid G) unitless
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Hannes Link, Bernd Anselment, Dirk Weuster-Botz, Leakage of adenylates during cold methanol/glycerol quenching
of Escherichia coli, Metabolomics (2008) 4: 240–247 DOI 10.1007/s11306-008-0114-6 p.245 right column 3rd paragraph |
| Method |
Pre-cultures were grown overnight in 1,000 ml shake-flasks
filled with 100 ml medium (37°C, 250 rpm). |
| Comments |
As the
adenylates energy charge is computed as: EC=([ATP]+[ADP]/2)/([ATP]+[ADP]+[AMP]) there would be no influence on the EC in case of unspecific
leakage (e.g., 70% loss of ATP, ADP and AMP). Using the
corrected values of adenylates found in this study the EC is
between 0.72 (quenching fluid H) and 0.59 (quenching
fluid G). Quenching fluid G=60% (v/v) methanol/H2O with 30 mM TEA. Quenching fluid H=60% (v/v)
methanol/glycerol with 30 mM TEA |
| Entered by |
Uri M |
| ID |
108129 |