||Bacteria Escherichia coli
||Hannes Link, Bernd Anselment, Dirk Weuster-Botz, Leakage of adenylates during cold methanol/glycerol quenching of Escherichia coli, Metabolomics (2008) 4: 240–247 DOI 10.1007/s11306-008-0114-6 link p.241 right column 6th paragraph
||P.241 right column top paragraph: "Pre-cultures were grown overnight in 1,000 ml shake-flasks filled with 100 ml medium (37°C, 250 rpm). All shake-flasks were inoculated by addition of 500µl culture from a glycerol stock."
||P.241 right column 6th paragraph: "Optical density (OD) was determined at 660 nm. The correlation of OD to cell dry weight was CDW = 0.56×OD660 g/l. Cell volume, cell size and cell number was estimated by a cell size analyser via the Coulter principle in a Multisizer II (Beckman Coulter, Krefeld,