| Value |
19
mV
|
| Organism |
Mouse Mus musculus |
| Reference |
H. S. Haraldsdottir, I. Thiele, & R. M. T. Fleming, Quantitative Assignment of Reaction Directionality in a Multicompartmental Human Metabolic Reconstruction, Biophysical J, February 17, 2012, supporting online material p.17/27 5th paragraphPubMed ID22768925
|
| Primary Source |
Koivusalo M, Steinberg BE, Mason D, Grinstein S. In situ measurement of the electrical potential across the lysosomal membrane using FRET. Traffic. 2011 Aug12(8):972-82. doi: 10.1111/j.1600-0854.2011.01215.x.PubMed ID21554506
|
| Method |
FRET |
| Comments |
A method for noninvasive measurement of lysosomal membrane potential, based on
fluorescence resonance energy
transfer (FRET), has recently been developed (28). Steady-state lysosomal membrane potential in mouse RAW264.7
macrophages averaged 19 mV. Evidence suggested that approximately half of this electrical potential was generated
by the activity of H+ V-ATPases in the lysosomal membrane, whereas the other half was generated by Donnan
e ffects. |
| Entered by |
Uri M |
| ID |
108104 |