Biomass concentration for different strains

Range TMB3001 0.530: TMB3001C5 0.581: TMB3001C1 0.479 gDW/OD unit
Organism Budding yeast Saccharomyces cerevisiae
Reference Sonderegger M, Sauer U. Evolutionary engineering of Saccharomyces cerevisiae for anaerobic growth on xylose. Appl Environ Microbiol. 2003 Apr69(4):1990-8. p.1991 right column 2nd paragraph from bottomPubMed ID12676674
Method P.1991 right column 6th paragraph: "Determination of physiological parameters: In batch cultures, exponential growth rates were determined by log-linear regression of OD600 versus time with growth rate as the regression coefficient. The specific biomass yield (YX/S) was determined from a plot of the coefficient of linear regression of the biomass concentration (X) versus substrate concentration (S) during the exponential growth phase. The biomass concentration was estimated from predetermined correlations between OD600 and cellular dry weight during the mid-exponential growth phase of aerobic cultures grown on glucose for strains TMB3001, TMB3001C5, and TMB3001C1 (0.530, 0.581, and 0.479 g/OD600 unit, respectively). During the exponential growth phase, specific glucose and xylose uptake rates were calculated by determining the ratio of the growth rate to YX/S. Ethanol, xylitol, acetate, and glycerol yields were calculated by linear regression of by-product concentration versus S."
Entered by Uri M
ID 107923