Maximal phage amplification rate and final ssDNA concentration in fed-batch processes with varying infection times

Range Table - link
Organism Bacteriophage M13
Reference Kick B et al., Specific growth rate and multiplicity of infection affect high-cell-density fermentation with bacteriophage M13 for ssDNA production. Biotechnol Bioeng. 2017 Apr114(4):777-784. doi: 10.1002/bit.26200 p.781 table IPubMed ID27748519
Method Abstract: "Here, the high-cell-density phage production with Escherichia coli as host organism was studied in respect of medium composition, infection time, multiplicity of infection, and specific growth rate."
Comments P.780 right column 2nd paragraph to p.781 left column top paragraph: "Infection with bacteriophage M13 was performed at different cell densities (15, 26, and 40 g /L, respectively) during the exponential feeding phase with an averaged multiplicity of infection (MOI) of 0.0025 ± 0.0012 pfu /cfu (Table I). Applying a poisson distribution, the proportion of cells infected with at least one phage can be calculated using the MOI (Ellis and Delbrück, 1938). At the time of infection, a minor proportion of 0.1–0.3% of the cells is theoretically infected. Despite the high standard deviation of the single MOIs, this indicates the comparability of the experiments. The addition of phages at a dry cell mass concentration of 40 g/L led to the highest maximal cell dry weight of 80 g/L, whereas the maximal phage titer reached the same magnitude up to 10^14 pfu/mL in comparison to the other infection times (Fig. 3A and B). Furthermore, the maximal phage amplification rate is independent of the time of infection, under the investigated conditions (Table I). The phage amplification rate describes the increase of phage titer in magnitudes per hour. For an early infection time, the stagnation in phage amplification rate occurred already during exponential feeding and is thus not caused by the end of exponentially growing cells (Fig. 3). Infection at 26 g/L after 5 h of exponential feeding resulted in the highest isolated ssDNA amount of 0.37 g/L (Table I)." See note beneath table
Entered by Uri M
ID 114092