| Range |
310 to 320 Table - link Genome copies/chloroplast
|
| Organism |
Wheat Triticum aestivum |
| Reference |
Boffey SA, Leech RM. Chloroplast DNA Levels and the Control of Chloroplast Division in light-grown wheat leaves. Plant Physiol. 1982 Jun69(6):1387-91. p.1390 table II and p.1391 left column 3rd paragraphPubMed ID16662409
|
| Method |
"The direct measurement of cpDNA/plastid used in this paper
relies on the isolation of plastid suspensions of known percentage
intactness, free from contamination by nDNA, and also on the
quantitative recovery of DNA from the isolated plastids. Sellden
and Leech (15) have shown that even the endogenous nucleases
in plastid preparations from wheat leaf regions beyond 4 cm from
the leaf base are sufficiently active to remove all contaminating
DNA." |
| Comments |
"The numbers of genome copies/chloroplast in the present paper
have been calculated on the assumption that the wheat chloroplast
genome is similar in size to those of other monocotyledons, so that
values presented must be regarded as estimates. The value for
mature wheat chloroplasts (310-320 genome copies/chloroplast)
compares with a figure for pea chloroplasts of 224±80 genome
copies/plastid (BNID 107108 ref 2, The published figures in Reference 2 are too low by a factor of 16, as
a result of an error in the use of the hemocytometer (J. Bennett, personal
communication).)" |
| Entered by |
Uri M |
| ID |
107107 |