| Range |
0.46 to 0.04 before lysis: 7.29 after cell lysis μm^2/sec
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Leptihn S, Har JY, Chen J, Ho B, Wohland T, Ding JL. Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria. BMC Biol. 2009 May 11 7: 22. p.6 of 13 left column bottom paragraphPubMed ID19432949
|
| Method |
Using fluorescence correlation spectroscopy (FCS), researchers measured in real-time the diffusion of 100
nM S1-Qdot conjugates upon binding to the immobilized
bacteria, which have been attached to a glass slide by
0.01% poly-L-lysine. |
| Comments |
At the end of 1 h, the
addition of SDS lysed the bacteria, releasing the AMPQdot
into the supernatant (Figure 4A). These particles
have D = 7.29 µm^2/sec which is comparable to that of free Qdot diffusion in solution, and approximately 18 times
faster than that of bound particles (fig.4B) |
| Entered by |
Uri M |
| ID |
106971 |