| Range |
5.9 to 21.0 Table - link 10^8cells/ml/OD
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Volkmer B, Heinemann M (2011) Condition-Dependent Cell Volume and Concentration of Escherichia coli to Facilitate Data Conversion for Systems Biology Modeling. PLoS ONE 6(7): e23126. doi:10.1371/journal.pone.0023126 p.2 table 1PubMed ID21829590
|
| Method |
P.3 left column 4th paragraph: "In order to enable the calculation of intracellular molar concentrations in a condition-dependent manner [Researchers] determined the OD-specific cell concentration by flow cytometry for different conditions and report this data as cell concentrations normalized
to the corresponding OD values (cells/ml/OD) to be able to compare the different conditions with each other." |
| Comments |
P.2 right column bottom paragraph: "Consistent with the literature, the length of the cells varied with the condition, between 1.6 µm for stationary cells and 3.9 µm for cells growing on LB medium (Table 1). Since E. coli cells grow by elongation, the cell length of individuals varies greatly in a population, thereby leading to a large standard deviation of the average cell length. Also in agreement with the literature, [researchers] found the cell width to be condition-independent (1.26 µm ±0.16 µm)." Table also has data for cells grown under stress conditions and different dilution rates. E. coli K-12 strain BW25113. Tests with the strain MG1655 on a few selected conditions revealed comparable results. Here, OD was measured at 600 nm. Note: OD readings might vary between different spectrophotometers. |
| Entered by |
Uri M |
| ID |
106578 |