| Range |
172 glucose plus amino acids medium: 173 glycerol medium µg/OD460 unit/ml
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Brunschede H, Dove TL, Bremer H. Establishment of exponential growth after a nutritional shift-up in Escherichia coli B/r: accumulation of deoxyribonucleic acid, ribonucleic acid, and protein. J Bacteriol. 1977 Feb129(2):1020-33. p.1021 right column 4th paragraphPubMed ID320174
|
| Method |
"The bacterial strain used was Escherichia Coli B/r (ATCC 12407). Cultures were grown at 37°C in minimal C medium (ref 15) supplemented with either 0.45% sodium succinate or 0.2% glucose or 0.2% glucose plus 20µg of each of the 20 standard L-amino acids per ml. Experimental cultures were inoculated by at least a 300-fold dilution of a fresh overnight culture containing the same nutritional supplements as the experimental culture. Growth was measured as the increase in the concentration of cell mass (absorbance at 460nm [A460] see below). The doubling times of the bacteria growing in the described media are listed in Table 1 (µ=Doublings per hour)." |
| Comments |
"The dry weight of (washed) bacteria was determined to be 172 and 173µg per A460 unit for bacteria grown in glucose plus amino acids or glycerol medium, respectively." |
| Entered by |
Uri M |
| ID |
106437 |