| Value |
1000
Proteins/mRNA/hour
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. p.339 left column bottom paragraph and right column top paragraph and fig.3b Corrected value is from Schwanhäusser B et al., Corrigendum: Global quantification of mammalian gene expression control. Nature. 2013 Mar 7 495(7439):126-7. doi: 10.1038/nature11848. p.126 right column 2nd paragraphPubMed ID21593866, 23407496
|
| Method |
Stable isotope labelling by amino acids in cell culture (SILAC). Newly synthesized RNA can be labelled with the nucleoside analogue 4-thiouridine (4sU). 4sU-containing mRNA can be purified and compared with the preexisting fraction to compute mRNA half-lives. |
| Comments |
NIH3T3 (mouse fibroblast) cells. On the basis of Ben-Tabou de-Leon S et al., (2009 PMID 19028486),
the estimated maximal translation rate constant in sea urchin embryos
is 140 copies per mRNA per hour, which is surprisingly close to the
prediction of researchers' model. |
| Entered by |
Uri M |
| ID |
106383 |