| Range |
100 to 10,000 proteins/mRNA
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Taniguchi Y, Choi PJ, Li GW, Chen H, Babu M, Hearn J, Emili A, Xie XS. Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science. 2010 Jul 30 329(5991):533-8. doi: 10.1126/science.1188308 p.537 left column 4th paragraphPubMed ID20671182
|
| Method |
Abstract: "[Investigators] carried out quantitative system-wide analyses of protein and mRNA expression in individual cells with single-molecule sensitivity using a newly constructed yellow fluorescent protein fusion library for Escherichia coli." |
| Comments |
P.537 left column 4th paragraph: "At the ensemble level, the mean mRNA abundances among these 137 genes range from 0.05 to 5 per cell, and are moderately correlated with the corresponding mean protein expression level at the gene-by-gene basis (correlation coefficient r = 0.77) (Fig. 3C). The lack of complete correlation, as reported previously in other organisms, is often attributed to differences in posttranscriptional regulation. Here, with the ability to determine the absolute number of molecules per cell, [investigators] determined the ratio between the mean protein abundance and the mean mRNA abundance to range from 10^2 to 10^4." See BNID 104186, 100196 |
| Entered by |
Uri M |
| ID |
106254 |