Number of proteins/mRNA

Value 5600 proteins/mRNA Range: 4,000 to 7,000 proteins/mRNA
Organism Budding yeast Saccharomyces cerevisiae
Reference Lu P, Vogel C, Wang R, Yao X, Marcotte EM. Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation. Nat Biotechnol. 2007 Jan25(1):117-24. abstract, p.120 caption of fig.4b,e and p.121 right column 2nd paragraphPubMed ID17187058
Method Abstract: "[Researchers] report a method for large-scale absolute protein expression measurements (APEX) and apply it to estimate the relative contributions of transcriptional- and translational-level gene regulation in the yeast and Escherichia coli proteomes. APEX relies upon correcting each protein's mass spectrometry sampling depth (observed peptide count) by learned probabilities for identifying the peptides."
Comments P.121 right column 2nd paragraph: "Further, binning the measurements (Fig. 4b) indicates that the relationship between the numbers of protein and mRNA molecules has the form of a power law with an exponent close to one. Therefore, the distribution of individual proteins is also well fit by a linear relationship of [protein] = 5,600 × [mRNA], implying ∼5,600 proteins present per mRNA, which is somewhat higher than previous estimates [refs 20,35]. The logarithm of the ratio of proteins per mRNA is well modeled (R^2 = 0.98) by a normal distribution (Fig. 4c)." See BNID 106202
Entered by Uri M
ID 104185