sNRP movement in nucleoplasm and splicing factor compartments of nucleus
| Range | Table - link |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, Shav-Tal Y, Neugebauer KM, Stanek D. The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells. J Cell Biol. 2010 Oct 4 191(1):75-86. p.78 table 1PubMed ID20921136 |
| Method | FCS (fluorescence correlation spectroscopy)-a technique that focuses a laser beam on a spot of interest in the nucleoplasm and monitors fluctuations in fluorescence intensity over time. The intensity record is transformed into an autocorrelation function that assesses the diffusion correlation time of the detected molecules. |
| Comments | HeLa cell lines that express from BACs the GFP-tagged snRNP proteins. |
| Entered by | Uri M |
| ID | 105699 |