Residence time of U2 and U5 snRNP with pre mRNA

Range 15-30 sec
Organism Human Homo sapiens
Reference Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, Shav-Tal Y, Neugebauer KM, Stanek D. The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells. J Cell Biol. 2010 Oct 4 191(1):75-86. p.82 right column top paragraphPubMed ID20921136
Method FRAP
Comments U1 and U4/U6 snRNP components exhibit fast dynamic exchange with a residence time of <1 s, whereas U2 and U5 snRNP proteins interact longer with pre-mRNA with a residence time of ~15–30 s
Entered by Uri M
ID 105698