| Value |
51.5
kJ/mole
Range: ±3.8 kJ/mole
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Schröder HC, Friese U, Bachmann M, Zaubitzer T, Müller WE. Energy requirement and kinetics of transport of poly(A)-free histone mRNA compared to poly(A)-rich mRNA from isolated L-cell nuclei. Eur J Biochem. 1989 Apr 15 181(1):149-58. p. 153 left column, bottom paragraphPubMed ID2565812
|
| Method |
Arrhenius analysis of the dependence of L-cell nuclear
envelope NTPase activity on temperature resulted in a linear
plot (temperature range 0-35°C) (p.154, Fig. 5B). The corresponding value for activation energy of total poly(a) rich mRNA was calculated to be 51.5±3.8 kJ/mol, suggesting a role of
NTPase in mediating nucleocytoplasmic RNA efflux. |
| Comments |
Isolated nuclei of mouse lymphoma L5178y cells. In vitro system shown to be an adequate model for in vivo mRNA transport (see p. 156 left column 3rd paragraph). See BNID 105620 |
| Entered by |
Uri M |
| ID |
105621 |