Time for ovary gland cell to externalize an amount of golgi vesicle membrane equivalent to the total plasma membrane

Value ~10 min
Organism Plant Aptenia cordifolia
Reference Kristen U, Lockhausen J. Estimation of Golgi membrane flow rates in ovary glands of aptenia cordifolia using cytochalasin B. Eur J Cell Biol. 1983 Jan29(2):262-7.PubMed ID6832170
Method Ovary gland cells of Aptenia cordifolia were exposed to 100 micrograms/ml cytochalasin B (cyt B) for 30 or 60 min during the phase of granulocrine polysaccharide secretion. The drug caused a congestion of Golgi vesicles around the dictyosomes, probably resulting from an inhibition of the vesicle migration towards the plasma membrane. The ultrastructural feature of the Golgi apparatus in control and cyt B treated cells was analyzed using stereological methods in order to estimate the mean area of vesicular membrane produced by a single dictyosome during a 30 min period of effective cyt B action.
Comments Assuming that the rate of vesicle congestion can be equated with the rate of vesicle production, the 236 dictyosomes found to be present in the non-growing ovary gland cells form 7517 vesicles in total, or approximately 32 vesicles each within a period of 30 min. This corresponds to a membrane turnover rate of 70.4 micrometers/min (this equals approximately 10% of the total plasma membrane area per min (hence value of 10 min in this BNID)), since the mean vesicle surface area was calculated to be 0.281 µm^2. See BNID 105559, 105560, 105561, 105772, 105773, 105775
Entered by Uri M
ID 105562