Isotropical outward expansion rate of colony after 2 hours lag time

Value 3.9 µm/sec
Organism Bacteria Bacillus subtilis
Reference Zhang HP, Be'er A, Florin EL, Swinney HL. Collective motion and density fluctuations in bacterial colonies. Proc Natl Acad Sci U S A. 2010 Jul 19.PubMed ID20643957
Primary Source Kearns DB, Losick R. Swarming motility in undomesticated Bacillus subtilis. Mol Microbiol. 2003 Aug49(3):581-90.PubMed ID12864845
Method Colonies grow on soft (0.5%) LB agar substrates. For inoculation, 5 µL of B. subtilis overnight culture (OD650 = 1) is placed on the agar. The inoculated gel is stored in an incubator at 30?°C and 90% humidity. After a lag time of 2 h, a colony starts to expand outward isotropically with a speed 1.4 cm/hour (primary source).
Comments For values of 0.013 and 0.023µm/sec in E. coli and gram positive bacteria, respectively, see BNID 105542 and comments section therein.
Entered by Uri M
ID 105543