| Value |
0.013
µm/sec
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Hallatschek O, Hersen P, Ramanathan S, Nelson DR. Genetic drift at expanding frontiers promotes gene segregation. Proc Natl Acad Sci U S A. 2007 Dec 11 104(50):19926-30. p. 19927 right column, top paragraphPubMed ID18056799
|
| Method |
The two (immotile) strains of E. coli were grown
overnight in separate liquid cultures at 37°C in rich growth
medium (LB) supplemented with Ampicillin. After tenfold dilution in fresh medium, we mixed them to obtain cultures with
different proportions of the two kinds of cells. Droplets of
different sizes were then pipetted on LB-agar plates (1.5% Agar)
with Ampicillin, and grown at 37°C for up to 84 h. Population
growth and mutant distribution were monitored at regular time
intervals by means of a stereo microscope. |
| Comments |
Note-Value of 0.023µm/sec for radial colony growth rate of a gram positive bacterium is given by Nithyaja et al., Kinetics of Bacterial Colony Growth by Laser Induced Fluorescence, Laser Physics, 2009, Vol. 19, No. 3, pp. 468–472. p. 469 right column, bottom sentence, fig. 4 |
| Entered by |
Uri M |
| ID |
105542 |