| Range |
51% proteins, 19% carbohydrates, 10% lipids, and 20% nucleic acids
|
| Organism |
Cyanobacteria Synechocystis PCC 6803 |
| Reference |
Shastri AA, Morgan JA. Flux balance analysis of photoautotrophic metabolism. Biotechnol Prog. 2005 Nov-Dec21(6):1617-26. p. 1620 left column, bottom paragraphPubMed ID16321043
|
| Method |
Autotrophic Growth Conditions. A 1-L culture of
Synechocystis was cultivated autotrophically in a 1.25-L
bioreactor (Bioflo 3000, New Brunswick Scientific, NJ)
under a surface light flux of 150 µE/m^2/s in BG-11
medium (American Type Culture Collection medium 616)
buffered with 10 mM HEPES under atmospheric levels
of carbon dioxide at 30°C. The pH was controlled at 7.5±0.1. Growth was monitored spectrophotometrically by
measuring the optical absorbance of cultures at 600 and
730 nm. |
| Comments |
The nucleic acids
were divided into 17% RNA and 3% DNA constituents (Neidhardt, F. C. Ingraham, J. L. Schaechter, M. Physiology
of the Bacterial Cell. A Molecular Approach Sinauer
Associates, Inc.: Sunderland, MA, 1990.) The final carbon and nitrogen balances
were within 1.8% and 6.5% of the elemental analysis (see BNID 105530). |
| Entered by |
Uri M |
| ID |
105531 |