| Value |
6.3
min
Range: ±0.4 Table - link min
|
| Organism |
Bacteria Clostridium perfringens |
| Reference |
Labbe R.G., Huang T.H. Generation times and modeling of enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens in laboratory media and ground beef. J. Food Prot. 1995 58 (12) 1303–1306. value of 6.3min on page 1304 right column top paragraph and Table 1 page 1305 |
| Method |
The growth of two enterotoxin-positive (ent+) and two enterotoxin-negative (ent-) strains of Clostridium perfringens were examined in two laboratory media, fluid thioglycollate and fluid thioglycollate with beef, and in autoclaved ground beef stored at 37, 41, 43, 46, and 48°C. There was no association between enterotoxigenicity and optimum growth temperature, which varied depending on strain and medium. Experimentally obtained values for generation times at 43°C were as low as 8.7 and 6.3 min in thioglycollate both for ent+ and ent- strains respectively. In autoclaved ground beef the shortest generation times, 7.1 min and 9.2 min, were obtained at 41 and 46°C for two ent+ strains. For ent- strains in autoclaved ground beef a generation time of 6.6 min was observed at 43°C. Temperature optima obtained experimentally were the same as those obtained by the Gompertz model. |
| Comments |
FTG+B=fluid thioglycollate with beef. The rapid growth of the ent+ strains between 41 and 46°C underlines previous recommendations for proper holding and cooling of protein foods such as meat and poultry known to support the growth of this organism. |
| Entered by |
Uri M |
| ID |
105469 |