Percent of GFP signal emanating from the gfpfs (frameshift) strain compaired to gfpwt (as result of error in transcription)

Value 2.4 % Range: ±0.4 %
Organism Bacteria Bacillus subtilis
Reference Meyerovich M, Mamou G, Ben-Yehuda S. Visualizing high error levels during gene expression in living bacterial cells. Proc Natl Acad Sci U S A. 2010 Jun 22 107(25):11543-8. p.11545 left column top paragraph & supporting information fig.S4B top row right-most columnPubMed ID20534550
Method "[Researchers] designed an experimental system to visualize and quantify the rate of spontaneous errors in gene expression at a single-cell level in living Bacillus subtilis cells. [Their] strategy was to introduce mutations into the ORF of a chromosomally encoded gfp reporter allele, such that errors in protein production would yield functional GFP molecules, visible by fluorescence microscopy."
Comments (pp.11544-5:)"[Researchers] estimated the average amount of errors that occur during exponential growth. The GFP signal emanating from the gfpfs [frame shift] and gfpns [nonsense] mutant strains was quantified, averaged, and expressed as a percentage of the GFP signal detected in gfpwt cells...Strikingly, the gfpfs cells contained 2.4±0.4% GFP molecules, whereas gfpns contained 0.4±0.1% fluorescent protein molecules relative to gfpwt cells."
Entered by Uri M
ID 105465