Growth rate on bufferred peptone-salt medium

Value 14 min
Organism Bacteria Vibrio parahaemolyticus
Reference Twedt RM, Novelli RM. Modified selective and differential isolation medium for Vibrio parahaemolyticus. Appl Microbiol. 1971 Oct22(4):593-9. p. 594 table 2 Table - link PubMed ID4943272
Method Several strains were grown on mixtures of peptone, proteose peptone, and thiotone dissolved in 3% NaCl, 0.25%K2HPO4 in pH 7.2. Culture flasks were incubated at 35°C and 160 rev/min in a gyratory shaker (New Brunswick Scientific Co., New Brunswick, N.J.). Viable cell counts were prepared from serial dilutions, and turbidities of flasks were determined at appropriate intervals from 0 through 24 hr. The generation time for a given medium, determined from the maximum tangent of a semi-log plot of turbidity versus time, equaled the shortest time for doubling optical density. Duplicate determinations were averaged.
Comments The concentration of peptone that supported the most rapid growth of strains in the simple buffered salt medium was 2.0%.
Entered by Uri M
ID 105457