Number of aminoacyl-tRNA molecules in cells of various strains

Range 52000-64000 Copies/cell
Organism Bacteria Escherichia coli
Reference Jakubowski H, Goldman E. Quantities of individual aminoacyl-tRNA families and their turnover in Escherichia coli. J Bacteriol. 1984 Jun158(3):769-76. Table - link PubMed ID6373741
Method Radioactive pulse-labeling of small cultures with 3H amino acids. The fraction of 3H-aminoacyl-tRNA has been measured as base-sensitive material in total 3H-amino acid-labeled nucleic acid.
Comments The total number of molecules of aminoacyl-tRNA is slightly higher in wild-type strain K38 (6.4×10^4 molecules per cell) and in revertant of trpS9969 (6.1×10^4 molecules per cell) than in mutant trpS9969 (5.2×10^4 molecules per cell) and its parent Ymel strain (5.7×10^4 molecules per cell). See BNID 104999
Entered by Uri M
ID 105274